Product overview

• Name
  NMDA
• Short description
  Prototypic NMDA receptor agonist
• Biological description
  Prototypic NMDA receptor agonist which mimics the action of glutamate. Potent excitant.
• Biological action
  Agonist
• Purity
  >99%
• Our products in action
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Properties

• Chemical name
  N-Methyl-D-aspartic acid
• Molecular Weight
  147.13
• Chemical structure
  
• Molecular Formula
  C₅H₉NO₄
• CAS Number
  6384-92-5
• PubChem identifier
  22880
• SMILES
  CN[C@H](CC(=O)O)C(=O)O
• InChi
  InChI=1S/C5H9NO4/c1-6-3(5(9)10)2-4(7)8/h3,6H,2H2,1H3,(H,7,8)(H,9,10)/t3-/m1/s1
• InChiKey
  HOKKHZGPKSLGJE-GSVOUGTGSA-N
• MDL number
  MFCD00004226

Applications

• Application notes

  The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 μM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 μM with prominent currents at 50 μM. The actions of NDMA were fully blocked by D-AP5 (NMDAR antagonist) at 100 μM (see Fig 1 above).

   

  #Protocol 1: NMDA mediated whole-cell currents

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  • Neurons were held at -70 mV and continuously perfused with aCSF in the presence of AMPA and GABA receptor antagonists CNQX (10 μM) and Bicuculline (100 μM) respectively and Tetrodotoxin (1 μM) to reduce network activity.
  • NMDA currents were evoked by applying NMDA directly to the recording chamber during continuous perfusion.
    • To test the selectivity of NMDA to NMDA receptors the experiment was repeated within the same neuron in the presence of the NMDA receptor antagonist D-AP5 (100 μM). Under these conditions NMDA failed to induce a depolarising current.

Storing and Using Your Product

• Storage instructions
  Room temperature
• Solubility overview
  Soluble in water (100mM)
• Important
  This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.